Help for Pomelo's image



Contents:

  1. Image
  2. Standardization
  3. Scale
  4. Missing values
  5. Rows
  6. Comments



1. Image

This image is provided as another way of looking at Pomelo's results. Each row is a gene and each column is a sample.

Rows are ordered by increasing adjusted p-value. First row corresponds to the row with the smallest adjusted p-value according to Pomelo's results, next one is the second lower adjusted p-value and so on.

Columns are ordered by classes in alphabetical order.

If you have a row with the names of columns, that row must start with "#name". For example:

#name	g1	g2	g1	g1	g2
gene1	23.4	45.67		6	85.6
genW@	34		23		13
genX#	23	44	25.6	29.4	13.2	     

Adjusted p-value and FDR indep. value (adjusted p-value using the FDR procedure of Benjamini & Hochberg), are shown at the start of each row. See Pomelo's help (Results file section) for details.

Once the first image is displayed, you can generate as many images as you want by filling the form that is under the image. New images will be displayed in a new window.


2. Standardization

If the data you are working with is not standardized, you must answer "yes" in the checkbox "Do you want to standardize your data?" in order to normalize it. By default your data will not be standardized.

If you choose to standardize you data, our standarization will transform your data to mean 0 and standard deviation 1. See next for details.


3. Scale

If you decided not to standardize your data, the scale units will be your data units. For example, if your data are in log (base 2) of the ratios, the scale will be in the same units. This means that a value of +3 (strongest red) is eigth times more than a value of 0.

Otherwise, if you decide to standardize your data, the scale will be presented in sigma units.

The scale limits can be selected between ±3, ±2 or ±1. Any values, either standardized or not, higher or lower than those limits will be shown as the highest or lowest value respectively.


4. Missing values

Any missing value will be shown as a white rectangle in the image.


5. Rows

The number of rows displayed by default is 50. If you want to see more rows, you have to fill in the text box "Select number of lines to show". Due to browser restrictions, images with more than 650 rows will not be shown. In these cases you will find a link to download the image (save link as) to your computer where you will be able to see it with a common image viewer.


6. Comments

If you have any question or comment, send Juanma an email.


Last modified: Fri Mar 07 18:49:22 CET 2003